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目的 探讨白细胞介素-12(IL-12)诱导小鼠红白血病细胞产生的树突状细胞,对白血病特异性CTL细胞的诱导作用,及体内免疫后对抗肿瘤免疫应答的诱导效果.方法:采用4/小时~(51)Cr释放法,检测CTL杀伤活性;观察小鼠红白血病细胞来源的树突状细胞体内免疫冶疗后对肿瘤的抑制作用,采用了HE染色和电镜等技术,分析肿瘤组织的病理学变化.结果:IL-12诱导FBL-3细胞产生的DC,在体外可诱导出FBL-3细胞特异性的CTL.采用IL-12诱导FBL-3细胞产生的DC免疫C57 BL/6小鼠.体内诱导出的CTL杀伤活性明显高于对照组.实验组小鼠能够有效的抵抗野生型FBL-3细胞的再攻击;肿瘤生长受到明显的抑制,组织学观察显示,肿瘤局部有较多炎性细胞浸润和细胞坏死;透射电镜可观察到典型的凋亡征象,结论:IL-12诱导小鼠FBL-3红白血病细胞产生的树突状细胞,在体内外可诱导出FBL-3细胞特异性的CTL,体内免疫后可增强抗肿瘤免疫应答,该结果为白血病的免疫治疗提供了新的途径.
Objective To investigate the effect of interleukin-12 (IL-12)-induced dendritic cells from mouse erythroleukemia cells, the induction of leukemia-specific CTL cells, and the induction of anti-tumor immune responses after in vivo immunization. 4/hour ~(51)Cr release assay to detect CTL killing activity; To observe the inhibitory effect of dendritic cells derived from mouse erythroleukemia cells after immunotherapy in vivo, using HE staining and electron microscopy to analyze tumors Histopathological changes in the tissue. Results: IL-12 induces DCs produced in FBL-3 cells and induces FBL-3 cell-specific CTLs in vitro. IL-2 induces DC immunity in FBL-3 cells. C57 BL/ 6 mice. In vivo CTL killing activity was significantly higher than the control group. Experimental mice can effectively resist the re-attack of wild-type FBL-3 cells; tumor growth was significantly inhibited, histological observation showed that the tumor has More inflammatory cell infiltration and cell necrosis; transmission electron microscopy can be observed typical signs of apoptosis, Conclusion: IL-12 induced mouse FBL-3 erythroleukemia cells produced by dendritic cells, in vitro and in vivo can induce FBL- 3 Cell-specific CTLs, after immunization in vivo Enhance the anti-tumor immune response, which results immunotherapy of leukemia provides a new way for.