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[目的]探讨染矽尘大鼠血浆、肺组织匀浆NO、NOS的关系。[方法]采用气管注射染矽尘(40mg/只)方法建立动物模型;血浆、肺组织匀浆NO浓度测定采用硝酸还原酶法;血浆、肺匀浆NOS活性测定采用NOS催化L—Arg法;采用SPSS8.0统计软件进行t检验和相关分析。[结果]矽尘可导致大鼠血浆NO水平升高(P<0.05)和NOS活力降低(P<0.05)、肺匀浆NO水平(P<0.05)和NOS活力均升高(P<0.01)。实验组血浆NO和血浆NOS有负相关关系(r=-0.775,P<0.05);血浆NOS和肺匀浆NO有负相关关系(r=0.800,P<0.05)。[结论]矽尘可导致大鼠血浆、肺匀浆NO水平和NOS活力变化;矽尘可导致血浆NO和血浆NOS有负相关关系,血浆NOS和肺匀浆NO有负相关关系。
[Objective] To investigate the relationship between NO and NOS in plasma and lung homogenates of rats exposed to silica dust. [Methods] Animal models were established by intratracheal instillation of silica dust (40mg / mouse). Nitric acid reductase was used to measure NO concentration in plasma and lung homogenate. L-Arg method was used to determine NOS activity in plasma and lung homogenate. Using SPSS8.0 statistical software for t test and correlation analysis. [Results] Silica dust could lead to the increase of plasma NO level (P <0.05) and NOS activity (P <0.05), the NO level of lung homogenate (P <0.05) and the increase of NOS activity (P <0.01) . There was a negative correlation between plasma NO and plasma NOS in the experimental group (r = -0.775, P <0.05). There was a negative correlation between plasma NOS and lung NO (r = 0.800, P <0.05). [Conclusion] Silica dust can cause NO level and NOS activity in plasma and lung homogenate of rats. Silica dust can cause negative correlation between plasma NO and plasma NOS and negative correlation between plasma NOS and lung homogenate NO.