论文部分内容阅读
目的 :观察中药调肝导浊汤对家兔实验性AS模型肝脏脂代谢及体外培养肝细胞膜脂蛋白受体的影响 ,探讨中药防治AS的机制。方法 :高胆固醇饮食饲喂家兔 10W造成AS模型 ,给药 6W结束实验 ,取肝脏匀浆测定TC、TG变化。此外 ,体外培养大鼠肝细胞 ,原位杂交法计数膜LDL受体阳性表达。结果 :调肝导浊汤可有效降低肝组织脂质含量 ,中药可以显著增强肝细胞膜LDL受体mRNA表达。结论 :调肝导浊汤具有良好的保护肝脏、调整脂代谢的作用 ,从而防治AS。
Objective : To observe the effect of Chinese medicine Tiaogandaozhuo decoction on hepatic lipid metabolism in experimental AS model in rabbits and in vitro culture of lipoprotein receptors in hepatic cell membranes, and to explore the mechanism of TCM prevention and treatment of AS. METHODS: Rabbits fed a high-cholesterol diet for 10 hrs resulted in an AS model. At the end of 6 hrs of administration, liver homogenates were used to measure changes in TC and TG. In addition, rat hepatocytes were cultured in vitro and membrane LDL receptor positive expression was counted by in situ hybridization. Results: Tiaogandaozhuo decoction can effectively reduce the lipid content of liver tissue. Chinese medicine can significantly enhance the expression of LDL receptor mRNA in hepatic cell membrane. Conclusion : Tiaogandaozhuo decoction can protect the liver and regulate lipid metabolism, so as to prevent and treat AS.