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目的 :建立接骨增效胶囊中柚皮苷含量测定方法。方法 :采用高效液相色谱法 ,色谱柱为HyporsilODS柱 (4.6mm×15 0mm ,5 μm) ;流动相为甲醇 水 冰醋酸 (35∶6 5∶0 .3,pH3) ;紫外检测波长 2 83nm ;流速为 1.2ml·min-1。结果 :线性范围是 2～35 μg·ml-1;回收率为 97.0 1% ,RSD =3.11% (n =5 )。结论 :方法简便、快速、准确 ,可作为该制剂的质控方法。
Objective : To establish a method for determination of naringin in Jiegu Zengxiao Capsule. Methods: HPLC was used. The chromatographic column was Hyporsil ODS column (4.6mm×150mm, 5 μm). The mobile phase was methanol water glacial acetic acid (35∶6 5∶0.3, pH3); UV detection wavelength was 283nm. The flow rate is 1.2 ml·min-1. Results: The linear range was 2～35 μg·ml-1. The recovery was 97.0 1% and RSD was 3.11% (n =5). Conclusion : The method is simple, rapid and accurate. It can be used as a quality control method for this preparation.