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目的:探讨缺氧对脑神经细胞、血管内皮细胞和血管平滑肌细胞的损伤顺序及β-细辛醚干预后对上述损伤的影响。方法:观察β-细辛醚对PC12细胞、血管内皮细胞和血管平滑肌细胞缺氧损伤形态变化,配合以MTT法和流式细胞分析法对各种细胞缺氧损伤后的存活和凋亡时间及状况进行分析。结果:在缺氧0.5 h时神经细胞凋亡率达到40%,在缺氧1.5 h时血管内皮细胞和血管平滑肌细胞凋亡率达到40%。β-细辛醚0.015 mg/ml组,0.03 mg/ml和0.06 mg/ml组对PC12细胞、血管平滑肌细胞均有保护作用,且呈剂量依赖性;β-细辛醚0.015 mg/ml组对血管内皮细胞无保护作用;0.06 mg/ml和0.03 mg/ml组均有保护作用。结论:一定剂量的β-细辛醚具有保护脑神经细胞、血管内皮细胞和血管平滑肌细胞免受缺氧损伤,提高细胞存活率的作用。
OBJECTIVE: To investigate the effect of hypoxia on the damage order of cerebral nerve cells, vascular endothelial cells, and vascular smooth muscle cells and the effect of β-asarone intervention on these injuries. METHODS: Morphological changes of hypoxia-induced injury induced by β-asarone in PC12 cells, vascular endothelial cells and vascular smooth muscle cells were observed. The time of survival and apoptosis after hypoxic injury of various cells was assessed by MTT assay and flow cytometry analysis. The situation is analyzed. RESULTS: The apoptosis rate of neurons reached 40% at 0.5 h of hypoxia, and the apoptosis rate of vascular endothelial cells and vascular smooth muscle cells reached 40% at 1.5 h of hypoxia. The β-asarone 0.015 mg/ml, 0.03 mg/ml and 0.06 mg/ml groups had protective effects on PC12 cells and vascular smooth muscle cells in a dose-dependent manner; β-asarone 0.015 mg/ml Vascular endothelial cells had no protective effect; 0.06 mg/ml and 0.03 mg/ml groups had protective effects. Conclusion: A certain dose of β-asarone can protect brain nerve cells, vascular endothelial cells and vascular smooth muscle cells from hypoxia injury and increase cell survival rate.