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为了建立一套快速、受外界环境影响小、结果准确可靠的辣椒杂种纯度鉴定技术，以 ２ 个辣椒杂种湘研 ５ 号、湘研 １０ 号及其父母本为材料，使用 Ｍ Ｊ／ Ｐ Ｔ Ｃ—２００ 型 Ｐ Ｃ Ｒ 仪及 Ｇ Ｄ Ｓ—８０００ 型凝胶成像分析系统，建立了适合辣椒 Ｒａｎｄｏｍ ｌｙ Ａｍ ｐｌｉｆｉｅｄ Ｐｏｌｙｍ ｏｒｐｈｉｃ Ｄ Ｎ Ａ（ Ｒ Ａ Ｐ Ｄ）分析的 Ｐ Ｃ Ｒ 扩增反应的实验体系，从 ３９ 个随机引物中找到了 ２ 个可用于鉴定湘研 ５ 号、４ 个可用于鉴定湘研 １０ 号杂种纯度的引物。
In order to establish a set of rapid, accurate and reliable identification technology of pepper hybrids with little effect on the external environment and accurate results, two pepper hybrids, Xiangyan 5 and Xiangyan 10, and their parents were used as materials. M J / P T C -200 PCR instrument and G D S-8000 gel imaging analysis system, an experimental system for P C R amplification reaction suitable for Peptide Random Ly Am plified Polym orphic D N A (R A P D) analysis was established Two of 39 random primers were used to identify Xiangyan 5 and 4 primers were used to identify Xiangyan10 hybrid purity.