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为了解长期接触烧煤、石油液化气和木柴废气对家庭主妇健康的潜在性危害,我们进行了尿液浓缩物致突变性的研究。材料和方法在以烧煤为燃料的某县城关镇,以烧石油液化气的某炼油厂和以烧木柴为唯一生活燃料的某县林场,分别选择从事家庭炊事工作的主妇11、23和13人作为搜集尿液的对象。每人搜集一次晨尿100m1.按照尿液致突变性的检测方法进行。选用较为敏感的TA98和TA100两种菌株,按照Ames操作的标准方法,在加与不加S9的条件下,用平皿掺入法进行试验,当受试样品的回变菌落数为阴性对照的2倍以上,并具有浓度-反应关
To understand the potential hazards of long-term exposure to coal, liquefied petroleum gas and firewood to the health of housewives, we conducted a study of the mutagenicity of urine concentrates. Materials and Methods Housewives 11, 23 and 13 engaged in household cooking were selected respectively in Chengguan Town, a county where coal is used as fuel, a refinery burning liquefied petroleum gas, and a forest farm in a county where burning wood was the only fuel for livelihood People as the object of collecting urine. Morning urine collected per person 100m1. In accordance with the detection of urine mutagenicity. Select the more sensitive TA98 and TA100 two strains, according to Ames standard method of operation, with and without the addition of S9 by plate incorporation test, when the test sample back to the number of colony-negative control 2 times more, and has a concentration - reaction off