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目的 探讨内皮一氧化氮合酶(eNOS)基因内含子4的多态性与糖尿病肾病的关系,以及其在中国人、马来西亚人和印度人中的分布。方法 选择258例病程在10年以上的2型糖尿病患者作为观察对象,其中中国人150例、马来西亚人71例、印度人37例。从患者全血中提取DNA,然后,进行聚酶链反应(PCR)、克隆及测序。结果 以往认为该基因有2种等位基因(a和b),而本研究发现3种等位基因,并对第3种多态(等位基因c)基因进行了序列分析;统计分析显示,等位基因a、b和c均与糖尿病肾病无显著相关性。结论 eNOS基因内含子4上的多态性有3种等位基因;该基因多态性与糖尿病肾病无显著相关性。
Objective To investigate the relationship between polymorphism of intron 4 of endothelial nitric oxide synthase (eNOS) gene and diabetic nephropathy and its distribution in Chinese, Malaysian and Indian. Methods Two hundred and eighty patients with type 2 diabetes mellitus with a disease course over 10 years were selected as the observation subjects. Among them, 150 were Chinese, 71 were Malays and 37 were Indians. DNA was extracted from patient whole blood and then subjected to polymerase chain reaction (PCR), cloning and sequencing. The results in the past that the gene has two kinds of alleles (a and b), and this study found three kinds of alleles, and the third polymorphism (allele c) gene sequence analysis; statistical analysis, Alleles a, b and c were not significantly associated with diabetic nephropathy. Conclusion There are three alleles in polymorphism of intron 4 of eNOS gene. There is no significant correlation between the polymorphism and diabetic nephropathy.