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目的 :对比研究羟丁酸钠和异丙酚对原代培养大鼠心肌细胞的毒性作用。方法 :将经原代培养成活 4d后的大鼠心肌细胞分为 5组 ,每组 6孔。 C为对照组 ,其余 4组分别加入小剂量 (HL ,3× 10 - 3 m ol/L )与大剂量 (HH,3× 10 - 2 mol/L )羟丁酸钠组、小剂量 (PL ,3× 10 - 5 m ol/L )与大剂量 (PH,3× 10 - 4 mol/L )异丙酚组。各组均于实验开始后 8h终止反应 ,评定心肌细胞搏动功能、观察细胞形态学改变、测定心肌细胞酶及电解质变化。 结果 :与对照组相比 ,PH组搏动频率减慢 (P<0 .0 5 ) ,细胞形态学亦有相应变化 ,肌酸激酸释放量增加 (P<0 .0 5 ) ,碱性磷酸酶活性下降 (P<0 .0 5 )。而 HL ,HH及 PL组的上述指标均无明显变化。各组间电解质变化未见显著差异。 结论 :高浓度的异丙酚具有直接的心肌抑制作用 ,而羟丁酸钠及低浓度的异丙酚几无心肌细胞毒性作用
OBJECTIVE: To comparatively study the toxic effects of sodium oxybate and propofol on primary cultured rat cardiomyocytes. Methods: The primary cultured cardiomyocytes were divided into 5 groups, 6 holes in each group. C as the control group and the other 4 groups were treated with low dose (HL, 3 × 10-3 mol / L) and high dose (HH, 3 × 10-2 mol / L) , 3 × 10 - 5 m ol / L) and high dose (3 × 10 - 4 mol / L) propofol group. Each group was terminated at 8h after the start of the experiment, the cardiomyocyte cardiomyocyte function was assessed, morphological changes of the cells were observed, and enzyme and electrolyte changes of cardiomyocytes were measured. Results: Compared with the control group, the pulsation frequency of PH group was slowed down (P <0. 05) and the morphological changes of cells were also observed. The release of creatine acid increased (P <0.05) Enzyme activity decreased (P <0.05). The HL, HH and PL group of the above indicators did not change significantly. Electrolytes between groups showed no significant difference. CONCLUSION: Propofol at high concentration has a direct myocardial inhibitory effect, whereas oxybutynin and propofol at low concentrations have no cardiomyocyte cytotoxicity