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目的研究胃癌前病变及胃癌中细胞凋亡与ＦａｓＬ表达的关系，探讨细胞凋亡在胃癌发病中的作用．方法采用脱氧核糖核酸末端转移酶介导的ｄＵＴＰ缺口末端标记（ＴＵＮＥＬ）技术及免疫组化染色对正常胃粘膜１０例，慢性胃炎７２例和胃癌５０例中凋亡细胞及ＦａｓＬ表达状态进行原位观察和比较．结果萎缩性胃炎、Ⅰ、Ⅱ、Ⅲ型肠化生及异型增生的凋亡细胞指数（１２８％，１７０％，１４６％，９６％，８０％）显著高于正常粘膜（３４％）和癌（５８％）（ｔ＝５６１，７５７，５１８，４７１，４０３；ｔ＝４３３，６４３，４１５，３０７，２１１；Ｐ＜００１及Ｐ＜００５）；异型增生组织凋亡细胞指数（８０％）显著低于萎缩性胃炎和Ⅰ型、Ⅱ型肠化生（１２８％，１７０％，１４６％；ｔ＝２６７，４７３，２９２；Ｐ＜００５及Ｐ＜００１）；Ⅲ型肠化生的凋亡细胞指数（９６％）显著低于Ⅰ型、Ⅱ型肠化生（１７０％，１４６％；ｔ＝３６９，２１３；Ｐ＜００１及Ｐ＜００５）．正常胃粘膜无ＦａｓＬ表达，胃癌前病变及胃癌中均有不同程度的ＦａｓＬ表达．ＦａｓＬ表达阳性肠化生、异型增生和胃癌中的凋亡细胞指数（１９８％?
Objective To study the relationship between apoptosis and FasL expression in gastric precancerous lesions and gastric cancer, and to explore the role of apoptosis in the pathogenesis of gastric cancer. Methods Apoptotic cells and FasL expression status in 10 cases of normal gastric mucosa, 72 cases of chronic gastritis and 50 cases of gastric cancer were performed in situ using DNA telomerase-mediated dUTP nick end labeling (TUNEL) technique and immunohistochemical staining. Observe and compare. Results Apoptotic cell index (12.8%,17.0%,14.6%,9.6%,8.0%) of atrophic gastritis, type I, type II, type III intestinal metaplasia and dysplasia were significantly higher. In normal mucosa (34%) and cancer (58%) (t=561,757,518,471,403;t=433,643, 415,307,211; P<001 and P<005); apoptotic cell index (80%) was significantly lower in atypical hyperplasia than in atrophic gastritis and in type I and II Intestinal metaplasia (128%,170%,146%;t=267,473,292; P<005 and P<001); type III intestinal metaplasia The apoptotic cell index (96%) was significantly lower than that of type I and II intestinal metaplasia (170%, 146%; t=369, 213; P<001). P<005). There was no expression of FasL in normal gastric mucosa, but there were different degrees of FasL expression in gastric precancerous lesions and gastric cancer. Apoptotic cell index in positive intestinal metaplasia, dysplasia, and gastric cancer in FasL expression (19.8%?