,Effect of daidzin, genistin, and glycitin on osteogenic and adipogenic differentiation of bone marr

• 作 者: Xiang-hui LI Jin-chao ZHANG Sen-fang SUI Meng-su YANG
• 机 构: State Key Laboratory of Biomembrane, Department of Biological Sciences and Biotechnology, Tsinghua U
• 出 处: 中国药理学报（英文版）
• 发表日期: 2005年9期
• 关键词: daidzin genistin glycitin bone marrow stromal cells osteogenic differentiation a

Aim: To examine the effect of daidzin, genistin, and glycitin on the osteogenic and adipogenic differentiation of bone marrow stromal cells (MSC) and the adipogenic transdifferentiation of osteoblasts. Methods: MTT test, alkaline phosphatase (ALP) activity measurement, Oil Red O stain and measurement were employed.Results: Daidzin, genistin, and glycitin 1× 10-8, 5× 10-7, 1× 10-6, 5× 10-6, and 1× 10-5mol/L all promoted the proliferation of primary mouse bone MSC and osteoblasts.Daidzin 5× 10-7 mol/L and genistin 1 × 10-6 mol/L promoted the osteogenesis of MSC. Genistin 1×10-8, 5×10-7, 1×10-6, 5×10-6, and 1×10-5 mol/L and glycitin 1×10-8,1× 10-6, and 1× 10-5 mol/L inhibited the adipogenesis of MSC. Daidzin, genistin,and glycitin 1×10-8,5×10-7, 1× 10-6, 5× 10-6, and 1× 10-5 mol/L all inhibited the adipocytic transdifferentiation of osteoblasts. Conclusions: Daidzin, genistin, and glycitin may modulate differentiation of MSC to cause a lineage shift toward the osteoblast and away from the adipocytes, and could inhibit adipocytic transdifferentiation of osteoblasts. They could also be helpful in preventing the development of osteonecrosis.