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目的探讨黄芪改善低血糖所致神经内分泌反向调节(counterregulatory responses,CRR)受损的可能机制。方法手术留置大鼠颈静脉和颈动脉导管,并随机分为黄芪组和对照组;术后第3天起分别给予1.5 ml黄芪提取液、0.9%NaCl溶液,连续7天。其中第7天起反复低血糖法造模连续3天,第11天进行高胰岛素低血糖钳夹实验,取血样检测中心血糖、肾上腺素(Epinephrine)、去甲肾上腺素(Norepinephrine)、肾上腺皮质激素(Cortisol)、胰高糖素(Glucagon);中枢糖敏感区域下丘脑室旁核(PVN)免疫组织化学染色,观察c-fos的表达。结果黄芪干预后急性低血糖时血糖轻度降低,对基础血糖并没有影响。黄芪组Epinephrine、Cortisol较对照组有显著升高。黄芪组PVN部位c-fos表达明显高于对照组。结论黄芪能增加低血糖时部分反向调节激素的分泌,可能与激活下丘脑糖敏感神经细胞的活性有关。
Objective To investigate the possible mechanism of Astragalus membranaceus for improving the damage of hypoxemia-induced neuroendocrine response (CRR). Methods The jugular and carotid ducts of rats were surgically removed and randomly divided into Astragalus group and control group. 1.5 ml Astragalus extract and 0.9% NaCl solution were given respectively on the 3rd day after operation for 7 consecutive days. The hypoglycaemia model was established on the 7th day for 3 consecutive days. On the eleventh day, the hyperinsulinemic hypoglycemic clamp experiment was performed. Blood samples were collected for blood glucose, epinephrine, norepinephrine, adrenocortical hormone Cortisol, Glucagon, and immunohistochemical staining of paraventricular hypothalamic paraventricular nucleus (PVN) in the central glucose-sensitive region. The expression of c-fos was observed. Results Astragalus acute hypoglycemia after intervention, slightly lower blood glucose, the basic blood sugar did not affect. Astragalus group Epinephrine, Cortisol significantly higher than the control group. Astragalus PVN c-fos expression was significantly higher than the control group. Conclusion Astragalus can increase the secretion of some anti-regulated hormones in hypoglycemia, which may be related to the activation of the sugar-sensitive neurons in the hypothalamus.