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研制一种基于荧光特性随PH值或金属离子浓度而变化的固定试剂的传感器,是当前人们很感兴趣的一个课题。为此,我们首先研究了基于固定荧光素胺的PH计。之所以作这种选择是因为它的操作简便,胺的化合物易于固定于固态载体上,而且,荧光素是一种有效荧光团。此外,酸碱反应很快,而其动态变化较慢,不会出现复杂的情况。这种基于固定荧光素试剂的PH计是具有生命力的,并且我们已经找到了它的某些特性。试验情况
The development of a sensor based on the immobilized reagent whose fluorescence characteristics change with the pH value or the metal ion concentration is a topic that people are currently very interested in. To do this, we first investigated the pH-based fixtures based on fluorescein amine. The reason for this choice is because of its ease of operation, the amine compound is easily immobilized on a solid support, and that fluorescein is an effective fluorophore. In addition, acid-base reaction quickly, and its dynamic changes slowly, will not appear complicated situation. This fixed fluorescein based pH meter is viable and we have found some of its properties. Test situation