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目的 探讨放射免疫导向手术在检测小鼠肺腺癌动物模型肿瘤转移的灵敏度、特异性及可靠性。方法 应用抗人肺腺癌单克隆抗体LC 1生物素化预定位技术 ,进行肺腺癌小鼠放射免疫导向手术。结果 γ照相机体外显像和井型探测仪体内放射性分布探测的结果显示 :在注射放射性核素 2h后 ,生物素化抗体预定位的荷瘤小鼠的肿瘤即有明显放射性浓集 ,其原发肿瘤和肺部转移灶的T/NT比值均显著高于未进行生物素化抗体预定位的荷瘤小鼠 (P <0 .0 1)。应用手持型γ探测仪 (GDP)对荷瘤小鼠进行体内探测 ,判别小鼠肺部肿瘤转移的灵敏度为 96% ,特异性为 98% ,准确率为 97%。结论 采用抗肺腺癌单克隆抗体LC 1生物素化预定位技术能明显提高小鼠肿瘤的放射性浓集 ,应用GDP进行小鼠体内探测可以探测出肿瘤及肺部微小转移灶
Objective To investigate the sensitivity, specificity and reliability of radioimmunoassay in the detection of tumor metastasis in mouse lung adenocarcinoma model. Methods The anti-human lung adenocarcinoma monoclonal antibody LC 1 biotinylated pretargeting technique was used to perform radioimmunization in mice with lung adenocarcinoma. Results The results of gamma camera in-vitro imaging and well detector detection in vivo revealed that the tumor of tumor-bearing mice pre-localized with biotinylated antibody had obvious radioactive concentration after 2h injection of radionuclide, T / NT ratios of tumors and lung metastases were significantly higher than those of tumor-bearing mice without biotinylated antibody pre-localization (P <0.01). In vivo detection of tumor-bearing mice using a hand-held gamma detector (GDP), the sensitivity of differentiating lung tumors in mice was 96%, specificity was 98% and accuracy was 97%. Conclusion The biotinylated pretargeting technique of anti-lung adenocarcinoma monoclonal antibody LC 1 can significantly increase the radioactive concentration of tumor in mice. Using GDP to detect in vivo mice can detect tumor and lung micrometastases