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作者采用犬冠状动脉灌流的方法 ,以犬颈动脉插管取血 ,经恒流泵从左旋支或前降支主干插管灌入 ,利用恒流泵来控制冠脉流量 ,实现不同程度心肌缺血的实验模型。经测定犬的主动脉压力与左旋支主干压力基本一致(t检验无显著差异 ) ,且主动脉压力较左旋支分支压力高出 1.30 k Pa,因此知道了正常主动脉压力 ,即可确定正常的冠脉压力或冠脉分支小动脉压力 ,同时又可确定正常的冠状动脉血流量。此方法不影响正常状态下的心脏功能 ,用恒流泵的控制旋扭按要求增加或减少灌流量 ,从而建立不同程度的心肌缺血模型。本实验方法不仅成本低 ,而且减少了中间环节 ,使实验的稳定性和准确性都有较大提高 ,并可进行定量分析。
The author uses canine coronary perfusion method, to take canine carotid artery cannulation, constant flow pump from left anterior descending artery catheterization, the use of constant flow pump to control coronary flow, to achieve varying degrees of myocardial ischemia Experimental model of blood. The aortic pressure was found to be approximately the same as that of the left circumflex artery (t test was not significantly different) and the aortic pressure was 1.30 kPa higher than the left branch pressure so that normal aortic pressure was known to determine normal Coronary pressure or arterial branch artery pressure, and at the same time to determine the normal coronary blood flow. This method does not affect the normal state of cardiac function, constant flow pump control knob required to increase or decrease perfusion, so as to establish different degrees of myocardial ischemia model. The experimental method not only low cost, but also reduce the intermediate links, so that the stability and accuracy of the experiment have greatly improved, and quantitative analysis.