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纯化的大豆胰蛋白酶抑制剂Ｔｉａ和Ｔｉｂ分别经烷基化、ＣＮＢｒ裂解、普通ＰＡＧＥ电泳、Ｔｒｉｃｉｎｅ／ＳＤＳ－ＰＡＧＥ电泳分析，初步证明Ｔｉｄ的突变位点发生在蛋白Ｎ端１～８４氨基酸残基上．
Purification of soybean trypsin inhibitor Tia and Tib by alkylation, CNBr cleavage, ordinary PAGE electrophoresis, Tricine / SDS-PAGE electrophoresis analysis showed that Tid mutation sites occur in the N-terminal amino acid residues 1 to 84 .