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目的了解四川省艾滋病病毒Ⅰ型(HIV-1)流行毒株的亚型、人群和地域分布。方法通过抽样调查的方法,采集2014年7-9月新报告的、未经抗病毒治疗的HIV-1感染者的血样400份,采用巢式聚合酶链反应(Nested PCR)扩增HIV-1 pol和gag基因区,对获得的核酸序列进行系统进化分析。比较不同亚型毒株感染者首次随访的CD4~+T淋巴细胞(简称CD4细胞)水平。结果四川省HIV-1流行毒株的亚型有:CRF07_BC(58.22%)、CRF01_AE(29.50%)、CRF08_BC(4.96%)、CRF85_BC(3.39%)、B(2.09%)、C(0.78%)、CRF02_AG(0.52%),以及CRF07_BC/CRF01_AE重组型(0.52%)。毒株分布显示出明显的地域特征。CRF01_AE感染者的基线CD4细胞水平更低。结论以C亚型为骨架的BC重组亚型,在四川省表现出明显的流行优势。提示应结合流行毒株有针对性地提供艾滋病防治工作建议。
Objective To understand the subtype, population and geographical distribution of HIV-1 strains in Sichuan province. Methods A total of 400 blood samples were collected from non-HIV-infected HIV-1 newly reported patients in July-September 2014 through a sample survey. Nested PCR was used to amplify HIV-1 pol and gag gene regions, the obtained nucleic acid sequence phylogenetic analysis. The levels of CD4 ~ + T lymphocytes (CD4 cells) in the first follow-up were compared among patients with different subtype infections. Results The subtypes of HIV-1 strains in Sichuan province were CRF07_BC (58.22%), CRF01_AE (29.50%), CRF08_BC (4.96%), CRF85_BC (3.39%), B CRF02_AG (0.52%), and CRF07_BC / CRF01_AE recombinant (0.52%). Strain distribution showed obvious regional characteristics. Patients with CRF01_AE had lower baseline CD4 cell levels. Conclusion The BC subtypes with C subtype as the skeleton show obvious epidemiological advantages in Sichuan Province. It is suggested that HIV / AIDS prevention and treatment work should be provided in a targeted manner in combination with epidemic strains.