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弓浆虫感染可诱导产生对该寄生虫的体液免疫应答及细胞免疫应答,其中细胞免疫是宿主抗弓浆虫感染的主要因素。为了研究过继转移的主要T 细胞亚群抗弓浆虫感染的能力,以弓浆虫RRA 株感染10～12周龄BALB/c 雄性小鼠,8个月后无菌取感染鼠及未感染鼠脾,用RPMI1640制成脾细胞悬液,以尼龙/羊毛纯化T 细胞,调节T 细胞浓度为10~7个/ml,加入抗CD_4单克隆抗体或抗CD_8单克隆抗体,在冰上作用40分钟,洗涤后加入兔补体,37℃孵育40分钟,洗涤后再制成悬液,经尾静脉给受鼠注入10~7细胞,24小时后,以弓浆虫感染—腹腔注射20个弓浆虫孢囊。
Toxoplasma gondii infection can induce humoral and cellular immune response to the parasite, of which cellular immunity is the main factor of host resistance to Toxoplasma infection. In order to investigate the ability of adoptive transfer of major T cell subsets against T. gondii infection, BALB / c male mice aged 10-12 weeks were infected with Toxoplasma gondii RRA strain. After 8 months, Spleen, the spleen cell suspension was made with RPMI1640, T cells were purified with nylon / wool, the T cell concentration was adjusted to 10 ~ 7 / ml, anti-CD_4 monoclonal antibody or anti-CD_8 monoclonal antibody was added to act on ice for 40 minutes After washing, rabbit complement was added and incubated for 40 minutes at 37 ° C. After washing, a suspension was made and 10 to 7 cells were injected into the tail vein to the mice. Toxoplasma infection Cysts.