论文部分内容阅读
以萘二甲酰亚胺与罗丹明B酰肼基硫脲两种有机荧光染料的光谱重叠特性,及罗丹明B酰肼基硫脲在p H诱导下的可逆“开-闭”环反应为基础,分别将其作为荧光共振能量转移(Fluorescence Resonance Energy Transfer,FRET)体系的供、受体,并与聚N-异丙基丙烯酰胺(PNIPAM)的温敏特性相结合,通过一系列化学反应,合成了一种具有双重荧光刺激-响应特性的大分子P(NIPAM-co-Rh BHA)-NP.采用1H NMR、FTIR、UV-vis和GPC对其结构进行了表征.采用荧光光谱(PL)研究了缓冲溶液中该聚合物对环境温度和p H值的双重荧光响应行为,并对其影响机制进行了探讨.结果表明,酸性条件下大分子中Rh BHA和NP-NH2之间会发生荧光共振能量传递;p H值和环境温度变化对大分子P(NIPAM-co-Rh BHA)-NP的荧光发射具有显著影响.
The spectral overlap characteristics between two kinds of organic fluorescent dyes of naphthalimide and rhodamine B hydrazylthiourea and the reversible “open-close ” ring of rhodamine B hydrazyl thiourea induced by p H Reaction as the basis, respectively, as a fluorescence resonance energy transfer (Fluorescence Resonance Energy Transfer, FRET) system for the receptor, and poly N-isopropyl acrylamide (PNIPAM) temperature-sensitive properties combined with a series (NIPAM-co-Rh BHA) -NP was synthesized by chemical reaction and its structure was characterized by 1H NMR, FTIR, UV-vis and GPC.The fluorescence spectra (PL) was used to study the double fluorescence response of the polymer to ambient temperature and p H values in buffer solution, and the mechanism of its effect was discussed. The results show that under acidic conditions, the interaction between Rh BHA and NP-NH 2 Fluorescence resonance energy transfer occurs; p H value and ambient temperature have a significant effect on the fluorescence emission of macromolecule P (NIPAM-co-Rh BHA) -NP.