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目的检测急性肝衰竭大鼠血清中的胃动素(MTL)、瘦素(Leptin)、饥饿激素(ghrelin)的浓度,以探究急性肝衰竭时胃肠功能障碍的因素。方法采用D-氨基半乳糖急性肝衰竭大鼠模型,麻醉后下腔静脉采血,用酶联免疫(ELISA)法分别检测正常大鼠和急性肝衰竭大鼠血清中的胃动素、瘦素、饥饿激素的浓度,并对两组的浓度值进行比较分析。结果急性肝衰竭大鼠较正常对照组大鼠的饥饿激素浓度明显升高,差异有统计学意义(t值分别为-4.531、-7.210,P<0.05);急性肝衰竭大鼠较正常对照组大鼠的胃动素浓度明显升高,差异有统计学意义(t值分别为-2.064、-2.530,P<0.05);急性肝衰竭大鼠较正常对照组大鼠的瘦素浓度明显升高,差异有显著差异(t值分别为-3.550、-6.031,P<0.05)。结论急性肝衰竭大鼠血清中的胃动素(MTL)、瘦素(Leptin)和饥饿激素(ghrelin)明显升高,这可能是肝衰竭时胃肠功能障碍时出现的恶心、呕吐、厌油、腹胀的原因之一。
Objective To detect the concentration of motilin, leptin and ghrelin in the serum of acute hepatic failure rats to explore the factors of gastrointestinal dysfunction in acute liver failure. Methods D-galactosamine-induced acute hepatic failure rat model was established. Blood was collected from the inferior vena cava after anesthesia. The contents of motilin, leptin, Hunger hormone concentrations, and the two groups of concentration values were compared. Results Compared with the normal control rats, the levels of gonadal hormones in acute liver failure rats were significantly increased (t = -4.531, -7.210, P <0.05, respectively). Compared with the normal control rats The concentration of motilin in rats was significantly increased (t = -2.064, -2.530, P <0.05, respectively); the concentration of leptin in rats with acute liver failure was significantly higher than that in normal rats (T = -3.550, -6.031, respectively, P <0.05). Conclusions The levels of MTL, Leptin and ghrelin in serum of acute hepatic failure rats are obviously increased, which may be the symptoms of nausea, vomiting and greasy oil during gastrointestinal dysfunction in liver failure One of the reasons for bloating.