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目的:研究骨松健骨方对骨质疏松模型大鼠股骨骨密度、力学结构的影响,探讨骨松健骨方治疗骨质疏松的作用机制。方法:采用去势雌性大鼠作为骨质疏松模型,将其分为假手术组、模型组、雌二醇组、骨松健骨方低、中、高剂量组,假手术组、模型组灌胃生理盐水,雌二醇组灌胃雌二醇,骨松健骨方低、中、高剂量组灌胃不同剂量骨松健骨方,给药12周后,检测大鼠股骨的骨密度和骨矿含量,三点弯曲实验检测大鼠股骨的生物力学性能,Micro-CT检测股骨远端的骨小梁数目及形态等变化情况。结果:给药后,E_2组、骨松健骨方低、中、高剂量组大鼠体质量不同程度低于模型组(P<0.05或P<0.01),且呈剂量依赖性;E_2组和骨松健骨方中剂量、高剂量组均能显著抑制大鼠股骨BMD的下降(P<0.01),呈剂量依赖性;骨矿物含量(BMC)在各组之间未见明显变化;E_2组及骨松益强方中、高剂量组可不同程度地提高大鼠骨应力、弹性模量(P<0.05或P<0.01)及最大载荷(P<0.05)。结论:骨松健骨方可提高去势大鼠的骨强度,防止大鼠雌激素减少所引起的体质量增加,增加大鼠骨密度,改善骨显微结构,增加骨小梁数目,降低骨小梁间隙,增强骨骼生物力学性能,对去势大鼠的骨丢失具有较好的防治作用。
OBJECTIVE: To study the effect of “Gusongjiangyu Decoction” on the femur bone mineral density and mechanical structure of rats with osteoporosis, and to explore the mechanism of Osteoporosis Treated by “Songsong Gangu Decoction”. Methods: The ovariectomized female rats were used as osteoporosis model. The rats were divided into three groups: sham-operation group, model group, estradiol group, low-, medium- and high-dose osteoporosis group, sham operation group, Gastric saline and estradiol groups were given orally estradiol and low and medium dose and high dose of Gusongjiangyao Decoction in different doses. After 12 weeks of administration, Bone mineral content, three-point bending test to detect the biomechanical properties of rat femur, Micro-CT detection of distal femoral trabecular number and morphological changes. Results: After the administration, the body weight of rats in E 2 group and the low, medium and high dose of ZGZJ group were lower than those in model group (P <0.05 or P <0.01) The bone mineral content (BMC) in the middle dose and high dose groups of Boneongjianjiangyu Decoction significantly inhibited the decline of femoral BMD in a dose-dependent manner (P <0.01) (P <0.05 or P <0.01) and the maximum load (P <0.05) in the medium and high dose of Gusongyi Qiangfang group. Conclusion: Gusongjiangyao can increase the bone strength of ovariectomized rats, prevent the increase of body weight caused by the reduction of estrogen in rats, increase the bone density of rats, improve the microstructure of bone, increase the number of trabecular and reduce the bone Trabecular clearance to enhance the biomechanical properties of the skeletal bone in ovariectomized rats with good prevention and treatment.