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目的考察可卡因染毒对小鼠免疫功能的影响。方法 615纯系雄性小鼠20只,随机均分为实验组和对照组;实验组小鼠以30mg/kg盐酸可卡因每天1次进行腹腔连续注射,空白组给予相同量盐水同法注射盐水;第30d处死小鼠,测量体重与脏器系数,用四甲基偶氮唑蓝(MTT)法检测脾细胞、胸腺细胞与腹腔巨噬细胞(PMφs)的增殖活性;用生物活性检测法检测IL-1与IL-2的活性;用ELISA法检测IFN-γ与TNF-α含量。结果实验组小鼠脾细胞、胸腺细胞与PMφs的增殖反应明显降低;脾细胞培养上清液中IL-2与IFN-γ活性明显降低;PMφs培养上清中IL-1与TNF-α活性明显降低;体重、脾脏与胸腺系数也明显降低;上述指标较之对照组均有显著性差异(P<0.01)。结论在活体条件下可卡因染毒对小鼠免疫系统功能有明显抑制作用。
Objective To investigate the effects of cocaine on immune function in mice. Methods Twenty-one 615 male mice were randomly divided into experimental group and control group. The mice in the experimental group were injected intraperitoneally with cocaine hydrochloride 30mg / kg once a day, while the blank group were given the same amount of saline and saline injection. The mice were sacrificed on day 30 and the body weight and organ coefficient were measured. The proliferation activities of spleen cells, thymocytes and peritoneal macrophages (PMφs) were detected by MTT assay. The bioactivity of IL- 1 and IL-2. The contents of IFN-γ and TNF-α were detected by ELISA. Results The proliferative responses of spleen cells, thymocytes and PMφs in the experimental group were significantly decreased. The activities of IL-2 and IFN-γ in the supernatant of spleen cells were significantly decreased. The activities of IL-1 and TNF-α in the supernatant of PMφs were significantly increased Decreased; body weight, spleen and thymus coefficient also significantly reduced; the above indicators compared with the control group were significantly different (P <0.01). Conclusions Cocaine exposure in vivo has a significant inhibitory effect on the immune system in mice.