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研究了微波辅助皂化－气相色谱－质谱联用（ＧＣ－ＭＳ）测定血清中胆固醇的分析方法。用０．４ｍｏｌ／ＬＫＯＨ－甲醇溶液为皂化液，在６００Ｗ微波功率下接受辐射３０ｓ皂化血清样品，选正庚烷为提取液。通过与水浴皂化法、酶学分光光度法比较，表明该法快速、溶剂用量少、准确、重现性好，方法的检出限为０．０１１ｍｇ，特别适用于生物、食品、体液等大量样品中胆固醇的测定，可快速为临床医学及生物工程等提供诊断数据。
The analytical method for the determination of cholesterol in serum by microwave-assisted saponification-gas chromatography-mass spectrometry (GC-MS) was studied. With 0.4mol / L KOH-methanol solution as the saponification solution, under the microwave power of 600W 30h exposure to saponification serum samples were selected n-heptane extract. Compared with water bath saponification and enzymatic spectrophotometry, the method showed that the method was rapid, the amount of solvent was less, accurate and reproducible. The detection limit of the method was 0.011 mg, which was especially suitable for mass production of biological, food, Determination of cholesterol in the sample, can quickly provide diagnostic data for clinical and biological engineering.