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目的：研究重组人肿瘤坏死因子（rhTNF-α）与重组白介素2 (rhIL2)协同体内抗鼻咽癌效应及抗瘤机理。方法：利用我室建立的鼻咽癌细胞株（CNE3）制成裸鼠鼻咽癌模型，用rhTNF-α或与rhIL2联合瘤内注射以观察抑瘤效应及荷瘤组织细胞的病理学、超微结构的改变，探讨其作用机理。结果：鼻咽癌裸鼠移植瘤经rhTNF-α治疗后肿瘤出现坏死、体积缩小、甚至消失。RhIL-2能增强rhTNF-α的抗瘤作用。组织病理学及超微结构观察发现，大多数癌细胞出现粗面内质网扩张；核碎裂、核溶解而消失。结论：rhIL-2增强rhTNF-α的抗瘤机理可能与IL-2诱导癌细胞表达TNF受体作用有关，本研究为临床在鼻咽镜下进行肿瘤局部注射TNF进行治疗提供实验依据。
Objective: To study the anti-nasopharyngeal cancer effect and anti-tumor mechanism of recombinant human tumor necrosis factor (rhTNF-α) and recombinant interleukin-2 (rhIL2) in vivo. Methods: Nasopharyngeal carcinoma cell line (CNE3) established in our laboratory was used to establish the model of nasopharyngeal carcinoma in nude mice. The antitumor effect was induced by rhTNF-α or rhIL-2 in combination with intratumoral injection to observe the pathology of tumor cells. Microstructural changes to explore its mechanism of action. Results: The tumor of nasopharyngeal carcinoma xenografts in nude mice after treatment with rhTNF-α showed necrosis, reduced in volume and even disappeared. RhIL-2 can enhance the anti-tumor effect of rhTNF-α. Histopathology and ultrastructure observed that most of the cancer cells appear rough endoplasmic reticulum dilatation; nuclear fragmentation, nuclear dissolution and disappear. CONCLUSION: The anti-tumor mechanism of rhIL-2 enhanced rhTNF-α may be related to IL-2-induced TNF receptor expression in cancer cells. This study provides experimental evidence for clinical treatment of tumor with TNF injection under nasopharyngectomy.