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我室三年来的小区试验已肯定芽孢杆菌(Bacillus sp.)处理小友种子有一定防病增产效果。为了进一步提高这种效果,有必要了解芽孢杆菌进入土中的消长规律,为此进行了系统观察。方法是将在牛肉汁培养48小时后含菌量每毫升为2000万个芽孢杆菌的菌液10毫升倒入20克无菌土挠匀后与150粒小麦种子拌匀(小麦种子经漂白粉消毒)。将拌种后的麦种分别播入无菌土和自然土中,40小时后开始测定根围含菌量,以后每天测一次。测定方法是每次取三粒麦种的根部,将根表土抖入5毫升无菌水中,收取0.4毫升容积的根表土,然后将根置无菌水中晃动几次,将无菌水加至10毫升,取出一毫升与无菌水按
Three years in my laboratory plot has confirmed the Bacillus sp (Bacillus sp.) Treatment of small seeds have a certain effect of disease prevention and increase production. In order to further improve this effect, it is necessary to understand the law of growth and decline of Bacillus into the soil, for which a systematic observation was conducted. The method comprises the following steps: 10 ml of bacterial liquid containing 20 million Bacillus subtilis after the 48 hours of beef juice culture is poured into 20 grams of sterile soil and evenly mixed with 150 seeds of wheat (the wheat seeds are bleached powder) . After the wheat seed dressing were sown sterile soil and natural soil, 40 hours after the determination of rhizosphere bacteria, measured once a day after. Determination of the method is to take three roots of wheat each time, the root soil into 5 ml of sterile water, charge 0.4 ml volume of root surface soil, and then the root of sterile water shaking several times, the sterile water was added to 10 Ml, remove one ml with sterile water press