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目的:通过观察高脂饮食诱导的肥胖倾向(DIO)及肥胖抵抗倾向(DIO-R)两种小鼠的血糖、血脂变化,探讨腺苷酸活化蛋白激酶活性水平在高脂饮食诱导小鼠肥胖易感性差异中的作用。方法:雄性ICR小鼠随机分为普通饮食对照组和高脂饮食组。喂养5周后,将高脂饮食组体重增加最大的10只判定为肥胖倾向小鼠,体重增加最小的10只判定为肥胖抵抗倾向小鼠。小鼠摘眼球取血后,检测血糖、血脂浓度。采用免疫印迹(Western Blot)法测定小鼠骨骼肌组织磷酸化的腺苷酸活化蛋白激酶α2(AMPKα2)、过氧化物酶增殖物激活受体γ共化合物1α(PGC-1α)、雌激素受体相关受体α(ERRα)和肉毒碱棕榈酰转移酶-1(CPT-1)蛋白表达。结果:(1)高脂饮食喂养5周后,DIO小鼠体重显著高于对照组和DIO-R小鼠(P<0.05)。(2)与普通饮食对照组小鼠相比,DIO小鼠和DIO-R小鼠血糖、血脂均有显著性差异(P<0.01),而DIO小鼠与DIO-R小鼠之间血脂四项无显著性差异。(3)高脂饮食喂养5周后,DIO小鼠骨骼肌pAMPKα2蛋白表达水平较普通饮食对照组显著减少(P<0.05)。结论:(1)对同一批小鼠采用同样高脂饮食喂养5周后,DIO和DIO-R小鼠体重增长显著不同,而血糖和血脂变化无明显差异。(2)在高脂饮食刺激下,小鼠骨骼肌AMPKα2活性差异可能导致骨骼肌内脂肪酸氧化水平不同,诱导出现DIO和DIO-R。
Objective: To observe the changes of blood glucose and blood lipid in obese proneness (DIO) and obesity-resistant (DIO-R) induced by high-fat diet and to investigate the effect of adenylate-activated protein kinase The role of differences in susceptibility. Methods: Male ICR mice were randomly divided into normal diet control group and high fat diet group. Five weeks after feeding, the 10 rats with the largest body weight gain in the high-fat diet group were judged as obese and the 10 with the smallest weight gain were judged as obesity-resistant mice. Mouse eyeball blood, blood glucose, blood lipid concentrations. Western blot was used to detect the phosphorylation of AMPKα2, PGC-1α, and estrogen in mouse skeletal muscle Related receptor α (ERRα) and carnitine palmitoyltransferase-1 (CPT-1) protein expression. Results: (1) Body weight of DIO mice was significantly higher than that of control and DIO-R mice (P <0.05) 5 weeks after feeding with high-fat diet. (2) Compared with the normal diet control group, the blood glucose and blood lipid of DIO mice and DIO-R mice were significantly different (P <0.01), while between DIO mice and DIO-R mice, No significant difference. (3) After fed with high-fat diet for 5 weeks, the expression of pAMPKα2 protein in skeletal muscle of DIO mice was significantly lower than that of normal diet control group (P <0.05). CONCLUSION: (1) The weight gain of DIO and DIO-R mice was significantly different from that of the same mice fed with the same high-fat diet for 5 weeks, but there was no significant difference in the changes of blood glucose and blood lipid. (2) The difference of AMPKα2 activity in skeletal muscle of mice stimulated by high-fat diet may lead to different levels of fatty acid oxidation in skeletal muscle and induce the appearance of DIO and DIO-R.