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以潮棕壤为研究对象,探讨了土壤浸提液冷冻干燥过程中调节pH、添加连二亚硫酸盐缓冲液(BD,0.11 mol L-1NaHCO3+0.11 mol L-1Na2S2O4)及二者组合对31P核磁共振(NMR)谱图分辨率的影响,试验设置对照CK、pH、BD、pH+BD添加等4个处理。结果发现,与对照和pH+BD处理相比,pH和BD单独处理31P NMR谱图分辨率更好。pH和BD单独处理比较,pH处理使谱图化学位移发生了较大的偏移,影响到土壤磷化合物的确定,而BD处理对谱图化学位移的影响较小。添加BD处理中,正磷酸盐、焦磷酸盐、多磷酸盐、单酯、磷脂、DNA等磷组分均可被检测出,而其他处理仅能检测出正磷酸盐、焦磷酸盐和单酯。总之,添加BD处理比其他处理较好地降低了谱图的化学位移偏移,提高了谱图分辨率,并检测出了较多的土壤磷组分。
Taking tidal brown soil as the research object, the effects of adjusting the pH during the freeze-drying process of soil extract, adding dithionite buffer solution (BD, 0.11 mol L-1 NaHCO3 + 0.11 mol L-1Na2S2O4) Nuclear magnetic resonance (NMR) spectral resolution of the impact of experimental settings CK, pH, BD, pH + BD added four treatments. As a result, it was found that the resolution of the 31P NMR spectrum of pH and BD alone was better than that of the control and the pH + BD treatment. Compared with the treatment with pH alone, the chemical shifts of the spectra were greatly offset by pH, which affected the determination of phosphorus compounds in the soil. However, the effects of BD treatment on the chemical shifts of the spectra were little. In BD treatment, phosphorus fractions such as orthophosphate, pyrophosphate, polyphosphate, monoester, phospholipid and DNA were all detected, while other treatments only detected orthophosphate, pyrophosphate and monoester . In conclusion, the addition of BD decreased the shift of chemical shifts better than other treatments, increased the spectral resolution and detected more soil P components.