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目的探讨褪黑素对脑出血大鼠应激性胃黏膜损伤的保护作用。方法将60只健康成年SD大鼠随机分为空白对照组、模型组、奥美拉唑组、褪黑素组。空白对照组、模型组大鼠给予生理盐水灌胃,奥美拉唑组以奥美拉唑悬液灌胃,褪黑素组给予褪黑素灌胃,预防性给药,连续灌胃7 d,末次给药后禁食水24 h,除了空白对照组外,其余3组均制作脑出血大鼠模型,通过酶联免疫吸附法检测大鼠血清中超氧化物歧化酶(superoxidedismutase,SOD)、丙二醛(malonaldehyde,MDA)含量,大体观察大鼠胃黏膜病变并计算溃疡指数(UI),HE染色光镜下观察胃黏膜组织形态学改变,并比较各组胃黏膜损伤的发病率。结果褪黑素的治疗增加了SOD含量,降低MDA含量、降低大鼠胃黏膜UI及胃黏膜损伤的发病率(P<0.05)。结论褪黑素可通过清除氧自由基而对应激性胃黏膜起到保护作用。
Objective To investigate the protective effect of melatonin on gastric mucosal injury induced by intracerebral hemorrhage in rats. Methods Sixty healthy adult SD rats were randomly divided into blank control group, model group, omeprazole group and melatonin group. The blank control group, the model group rats were given normal saline gavage omeprazole group omeprazole suspension gavage, melatonin group given melatonin gavage, prophylactic administration, continuous gavage 7 d , The fasting water after the last administration for 24 h, except for the blank control group, the other three groups were made intracerebral hemorrhage rat model by enzyme-linked immunosorbent assay serum superoxide dismutase (superoxide dismutase, SOD), C The content of malonaldehyde (MDA) was measured. The gastric mucosal lesion was observed and the ulcer index (UI) was calculated. The morphological changes of gastric mucosa were observed by HE staining and the incidence of gastric mucosal lesion in each group was compared. Results Melatonin treatment increased the content of SOD, decreased the content of MDA and decreased the incidence of UI and gastric mucosal injury in rats (P <0.05). Conclusion Melatonin can protect the stress gastric mucosa by removing oxygen free radicals.