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目的建立HPLC测定白花蛇舌草中熊果酸、异熊果酸和对香豆酸的含量。方法采用Diamonsil C18 ODS(4.6 mm×250 mm,5μm),流动相为乙腈-15 mmol·L?1 NH4Ac(12∶88,p H 3.5),流速为1.0 m L·min?1,检测波长为308 nm,柱温为35℃。结果熊果酸、异熊果酸和对香豆酸线性范围分别为0.075 7~2.271μg(R2=0.999 6),0.010 3~0.309μg(R2=0.999 2)和0.020 2~0.606μg(R2=0.999 6);3种成分测定的平均回收率分别为99.9%(RSD=0.7%),99.0%(RSD=1.7%)和101.1%(RSD=0.7%)。结论本法简便、准确度高、稳定性和重复性好,可用于白花蛇舌草中指标性成分定量分析和质量控制。
Objective To establish HPLC determination of ursolic acid, ursolic acid and p-coumaric acid in Hedyotis diffusa Willd. Methods Diamonsil C18 ODS (4.6 mm × 250 mm, 5 μm) was used. The mobile phase was acetonitrile-15 mmol·L -1 NH4Ac (12:88, p H 3.5) at a flow rate of 1.0 m L · min -1. The detection wavelength was 308 nm, column temperature 35 ℃. Results The linear range of ursolic acid, ursolic acid and p-coumaric acid were 0.075 7 ~ 2.271 μg (R2 = 0.999 6), 0.010 3 ~ 0.309 μg (R2 = 0.999 2) and 0.020 2 ~ 0.606 μg (R2 = 0.999 6). The average recoveries of the three components were 99.9% (RSD = 0.7%), 99.0% (RSD = 1.7%) and 101.1% (RSD = 0.7%), respectively. Conclusion This method is simple, accurate, good stability and reproducibility, and can be used for quantitative analysis and quality control of the index components in Hedyotis diffusa.