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以“富有”和“次郎”的休眠芽为外植体，对甜柿离体快繁技术进行了研究。结果表明，聚乙烯吡咯烷酮（ＰＶＰ）可有效地防止外植体褐变，提高分化率。芽的增殖和生长适宜的ＺＴ和ＩＡＡ浓度分别为１～２ｍｇ／Ｌ和０．０５～０．１０ｍｇ／Ｌ。在含有ＺＴ１ｍｇ／Ｌ的改良ＭＳ（ＫＮＯ３和ＮＨ４ＮＯ３减半）培养基中加入ＢＡ１ｍｇ／Ｌ，能提高有效新梢率，降低试管苗成本。ＩＡＡ诱导生根的效果优于ＩＢＡ，用生长素溶液浸新梢基部后转入１／２ＭＳＯ培养基中对生根更有利。在较低温度下，以腐殖土作基质，试管苗的移栽成活率可达８５％以上。
The dormant buds of “rich” and “Jiro” were used as explants to study the rapid propagation of sweet persimmon in vitro. The results showed that polyvinylpyrrolidone (PVP) could effectively prevent the explants from browning and improve the differentiation rate. The optimal concentrations of ZT and IAA for bud proliferation and growth were 1 ~ 2 mg / L and 0.05 ~ 0.10 mg / L, respectively. The addition of BA1mg / L to the modified MS containing ZT1mg / L (KNO3 and NH4NO3 halved) can increase the effective shoot rate and reduce the cost of plantlets. The rooting effect of IAA was better than that of IBA. It was more beneficial to rooting by soaking the shoot base with auxin solution and transferring it into 1/2 MSO medium. At lower temperature, with humus soil as a substrate, the transplanting survival rate of test tube seedlings can reach more than 85%.