担子菌LMPQ39和CSP菌丝原生质体分离和再生的研究

• 作 者: 陈晓舸 黄瑞珊 朱章玉
• 出 处: 上海交通大学学报
• 发表日期: 1994年02期
• 关键词: CSP LMPQ39 担子菌纲 菌龄 酶液 酶解温度 纤维素酶 脱壁 酶解试验 酶解条件

本文以蜗牛酶和纤维素酶的混和酶液作为脱壁酶，通过酶解试验比较了菌龄、酶解时间、酶解温度及酶液浓度等对担子菌ＬＭＰＱ３９和ＣＳＰ菌丝原生质体分离的影响．通过原生质体再生试验，比较了渗透压稳定剂对ＬＭＰＱ３９和ＣＳＰ菌丝原生质体再生的影响．获得了１０５～１０８数量级的原生质体并再生出菌落．同时，本文还对ＣＳＰ原生质体再生过程的形态变化作了适当的描述．

In this paper, snail enzyme and cellulase mixed enzyme solution as a wall-clearing enzyme, enzymatic hydrolysis test compared the bacteria age, enzymolysis time, enzymatic temperature and the concentration of the enzyme on the LMPQ39 and CSP protoplasts isolation of protoplasts influences. The effects of osmotic stabilizer on mycelial protoplast regeneration of LMPQ39 and CSP were compared by protoplast regeneration test. Protoplasts on the order of 105-108 were obtained and colonies regenerated. At the same time, this article also describes the morphological changes of CSP protoplast regeneration process.