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目的:探索用氨乙酸丙酸(ALA)对髓性白血病细胞进行光敏化灭活。方法:用荧光光谱技术测量ALA在白血病细胞中内源生成光敏药物原卟啉Ⅸ;用共焦激光扫描荧光显微镜结合亚细胞器荧光探针探测其在JCS髓性白血病细胞中的分布;用MTT方法检测光敏化后的细胞存活。结果:ALA成功的在白血病细胞中内源生成光敏药物原卟啉Ⅸ;原卟啉Ⅸ分布在细胞核以外的细胞质中,线粒体是其的结合部位之一;经光照后此内源生成的原卟啉Ⅸ启动光敏化反应有效的灭活白血病细胞。结论:基于ALA的光动力反应有潜力用于对白血病细胞的光敏化灭活。
Objective: To explore the photosensitization and inactivation of myeloid leukemia cells with amino acid propionic acid (ALA). METHODS: Fluorescence spectroscopy was used to measure the endogenous production of photosensitizing protoporphyrin IX by ALA in leukemia cells and its distribution in JCS myeloid leukemia cells was detected by confocal laser scanning fluorescence microscopy combined with subcellular fluorescence probes; the MTT method was used. The photoactivated cells were examined for survival. RESULTS: ALA successfully generated endogenous photosensitizing protoporphyrin IX in leukemia cells; protoporphyrin IX was distributed in the cytoplasm outside the nucleus, and mitochondria were one of its binding sites; this endogenously produced protozoan was irradiated with light. The lin IX initiates a photosensitizing reaction to effectively inactivate leukemia cells. Conclusion: Photodynamic reactions based on ALA have the potential to be used for the photoactive inactivation of leukemia cells.