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[目的]建立血细胞天然免疫反应能力的测定方法。[方法]采用将癌细胞加入新鲜枸橼酸抗凝血中 (或血细胞和血浆中 ) ,37℃孵育30分钟。[结果]发现血细胞 (红细胞、淋巴细胞、粒细胞 )粘附到肿瘤细胞形成花环状 ,肿瘤患者各种血细胞粘附癌细胞形成花环率明显低于正常人 (P<0.01)。[结论]血细胞粘附癌细胞形成花环状的试验可用于测定血细胞天然免疫反应能力 ,在临床上有一定的应用价值。
[Objective] To establish a method for determination of natural immune response of blood cells. [Methods] Cancer cells were added to fresh citrate anticoagulant (or blood cells and plasma) and incubated at 37 ° C for 30 minutes. [Results] Hematocytes (erythrocytes, lymphocytes, granulocytes) were found to adhere to the tumor cells to form the rosette. The rate of formation of rosette on the adherent cells of various blood cells in tumor patients was significantly lower than that in normal people (P <0.01). [Conclusion] The test of hematopoietic cell adhesion to cancer cells to form a rosette can be used to measure the natural immune response of blood cells and has certain clinical value.