论文部分内容阅读
目的:通过研究凝血常规及血常规分析前的影响因素,探讨控制分析前变异的方法和意义。方法:选择健康体检者21人,采血进行凝血常规和血常规项目的检测,观察止血带使用时间对凝血常规、标本放置时间对血常规等结果的影响,监测凝血常规及血常规的个体生物学变异。结果:1与止血带使用即刻相比较,压脉带使用1 min时,凝血酶原时间(PT)、部分凝血活酶时间(APTT)、纤维蛋白原(FIB)和凝血酶时间(TT)均无变化;2 min时,PT降低(P<0.05),余无变化;3 min时,PT、APTT、和TT均降低(P<0.05)。2与标本放置即刻的血常规结果比较,标本放置4 h时,红细胞数(RBC)、血红蛋白浓度(HGB)、白细胞数(WBC)和血小板数(PLT)均无变化;放置8 h和12 h时,PLT降低(P<0.05);放置24 h,RBC、WBC和PLT变化均有统计学意义。3连续4周测定,各周间凝血常规及血常规个体生物学变异的差异均无统计学意义。结论:分析前标本采集方式和标本放置时间会对检测结果造成影响,重视分析前影响因素和控制分析前变异,能极大地提高临床实验室检测数据的可靠性。
Objective: To explore the methods and significance of controlling pre-analysis variation by studying the influencing factors before coagulation and routine analysis. Methods: Twenty-one healthy subjects were selected for blood coagulation tests and routine blood tests. The effects of tourniquet time on blood clotting routine and specimen placement time on blood routine were monitored. The coagulation routine and blood routine individual biology variation. Results: 1 Compared with the immediate use of tourniquet, prothrombin time (PT), partial thromboplastin time (APTT), fibrinogen (FIB) and thrombin time (TT) PT did not change at 2 min (P <0.05), and remained unchanged. At 3 min, PT, APTT and TT decreased (P <0.05). (2) Compared with the instant blood routine results, there was no change in the number of RBC, HGB, WBC and PLT after being placed for 4 h; PLT decreased (P <0.05). After left for 24 hours, the changes of RBC, WBC and PLT were statistically significant. 3 measured for 4 weeks in a row, the clotting routine and blood routine individual biological differences in each week were not statistically significant. Conclusion: The pre-analysis method and sample placement time will affect the test results. Pay attention to pre-analysis factors and control pre-analysis variation, which can greatly improve the reliability of clinical laboratory test data.