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目的 探讨腺病毒介导的野生型p5 3在前列腺癌基因治疗中的可能性及其机制。 方法 培养前列腺癌细胞系PC3M ,转染腺病毒介导的野生型p5 3,检测其增殖和凋亡水平变化 ,并检测其对bax蛋白及mRNA水平的影响。 结果 腺病毒介导的野生型p5 3可高水平转染入PC3M细胞 ,转染率 95 %。转染后的PC3M细胞增殖受到抑制 ,BrdU吸光度A值 0 .4 96 ,显著低于对照组的 1.4 5 4 ,P <0 .0 0 1;细胞凋亡水平增高 ,凋亡指数 16 .2 5 % ,显著高于对照组的 3.6 0 % ,P <0 .0 0 1。细胞中bax蛋白及mRNA水平皆增高。 结论 腺病毒介导的野生型p5 3可抑制PC3M细胞增殖并促进其细胞凋亡 ,其作用可能是通过促进bax表达而实现的。
Objective To investigate the possibility and mechanism of adenovirus mediated wild-type p53 in gene therapy of prostate cancer. Methods Prostate cancer cell line PC3M was cultured and transfected with adenovirus-mediated wild-type p5 3. The proliferation and apoptosis of adenovirus p5 3 were detected by immunohistochemistry. The effect of bcl-2 on bax protein and mRNA was also examined. Results Adenovirus-mediated wild-type p5 3 transfected into PC3M cells at a high level showed 95% transfection efficiency. After transfection, the proliferation of PC3M cells was inhibited. The BrdU absorbance A value was 0.496, which was significantly lower than that of the control group (P <0.01). The apoptotic index increased 16.25 %, Which was significantly higher than 3.6% of the control group, P <0.001. Cell bax protein and mRNA levels were increased. Conclusions Adenovirus-mediated wild-type p5 3 can inhibit PC3M cell proliferation and promote its apoptosis, which may be through promoting bax expression.