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目的:探讨中国常州地区汉族人群血清白三烯(LT)B4水平、花生四烯酸5-脂氧合酶激活蛋白(ALOX5AP)基因SG13S89G/A多态性和急性心肌梗死(AMI)三者的相互关联性。方法:以262例AMI患者(AM I组)和132例非冠心病患者(对照组)作为研究对象,采用聚合酶链反应-限制性片段长度多态性方法检测ALOX5AP基因SG13S89G/A多态性,同时采用酶联免疫吸附试验检测血清LTB4水平(以中位数/四分位数间距表示)。结果:对照组与AMI组ALOX4AP基因SG13S89G/A位点的(AA+GA)基因型(7.58%︰4.96%)、GG基因型(92.42%︰95.04%)以及A等位基因(4.17%︰2.67%)频率均差异无统计学意义;多变量Logistic回归分析显示,该位点多态性与AMI发病风险无显著相关性;AMI组血清LTB4水平显著高于对照组(477.97/370.52pg/ml︰200.57/236.65pg/ml,P<0.01);多变量Logistic回归分析显示,血清LTB4水平与AMI发病风险有显著相关性(P<0.01);AMI组和对照组的组内(AA+GA)基因型和GG基因型之间的血清LTB4水平比较差异无统计学意义。结论:中国常州地区汉族人群AMI患者血清LTB4水平显著升高,ALOX5AP基因SG13S89G/A多态性与AMI易感性无关,且不影响血清LTB4水平。
Objective: To investigate the relationship between serum leukotriene (LT) B4 level and SGC-S89G / A polymorphism of arachidonic acid 5-lipoxygenase activating protein (ALOX5AP) gene and acute myocardial infarction (AMI) in Han nationality of Changzhou, Interrelatedness. Methods: Two hundred and sixty-two AMI patients (AMI group) and 132 non-CHD patients (control group) were enrolled in this study. The polymorphism of ALOX5AP gene SG13S89G / A was detected by polymerase chain reaction-restriction fragment length polymorphism Serum levels of LTB4 were measured by enzyme linked immunosorbent assay (median / quartile spacing). Results: The AA genotype (7.58%: 4.96%), GG genotype (92.42%: 95.04%) and A allele (4.17%: 2.67) in ALOX4AP gene SG13S89G / A locus in control group and AMI group %) Had no significant difference. Multivariate logistic regression analysis showed that there was no significant correlation between the polymorphism of AMI and the risk of AMI. The level of LTB4 in AMI group was significantly higher than that in control group (477.97 / 370.52pg / ml: Multivariate Logistic regression analysis showed that there was a significant correlation between the level of serum LTB4 and the risk of AMI (P <0.01); the intra-AA (AA +) genes in AMI and control groups There was no significant difference in the level of serum LTB4 between GG and GG genotypes. CONCLUSIONS: Serum levels of LTB4 are significantly elevated in AMI patients of Han nationality in Changzhou, China. The ALOX5AP gene SG13S89G / A polymorphism has no relation with AMI susceptibility, and does not affect serum LTB4 level.