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Genkwadane F—I(1-4),four new diterpenes,along with four known ones(5-8),were isolated from the flower buds of D.genkwa.The structures including absolute configurations of 1-4 were determined based on the analysis of NMR and ECD calcula-tions.All compounds were evaluated for their cytotoxicities against MCF-7 and Hep3B cell lines,among which compounds 3 and 4 showed significant activities.Compound 4 exhibited the most potent activity with ICs0 values of 1.33 μM against MCF-7,and the IC50 values of compound 3 against MCF-7 and Hep3B were 5.54 μM and 8.73 μM,respectively.Further studies by Hoechst 33258 staining and Annexin V/PI analysis demonstrated that compounds 3 and 4 could induce apoptosis to exhibit anti-tumor activities.