论文部分内容阅读
目的调查中俄边境黑瞎子岛地区鼠群斑疹伤寒立克次体的感染状况。方法于2013-2014年在黑瞎子岛地区采用夹夜法捕鼠,无菌取其肝脏组织,提取鼠肝脏总核酸,采用巢式聚合酶链反应(nest-PCR)技术,对提取的鼠肝脏总核酸中glt A基因的136 bp特异性片段进行扩增,对阳性扩增产物测序,并利用Mega 5.0软件与Gen Bank中已知菌株基因序列进行比对和系统发育分析。结果共捕鼠644只,隶属于3目5科8属,其中黑线姬鼠为优势鼠种,占捕获总数的42.70%(275/644),其次为红背鼠平和东方田鼠,分别占27.64%(178/644)和21.12%(136/644)。共检出31只鼠感染斑疹伤寒,阳性率为4.81%,分属于啮齿目中5种鼠,其中东方田鼠的感染率相对较高,为9.56%(13/136),其他啮齿目鼠形动物感染率相对较低,但各鼠种间感染率差异无统计学意义(P=0.156)。雌鼠的感染率(10/168,5.95%)虽高于雄鼠(21/476,4.41%),但差异无统计学意义(χ2=0.643,P=0.423)。斑疹伤寒感染组体重均值(38.2548 g)与非感染组的体重均值(32.2271 g)差异无统计学意义(F=1.633,P=0.202)。不同捕鼠时间鼠斑疹伤寒的感染率差异有统计学意义(χ2=191.419,P=0.000)。该地区鼠群感染的斑疹伤寒立克次体在同一进化支上,相似度>99.98%,与美国菌株CP003398在进化上最为相近,相似度>95.83%。结论黑瞎子岛地区鼠群存在斑疹伤寒立克次体感染,且感染状况存在季节性差异,为科学开发黑瞎子岛提供了依据。
Objective To investigate the infection status of rickettsia rickettsia in black blind island of China-Russia border. Methods In the area of Heiminzi Island from 2001 to 2014, the rats were caught by the method of catgut nocturnal and the liver tissues were aseptically taken out. The total nucleic acids in the liver of the rats were extracted. The extracted rat livers were obtained by nested-PCR, The 136 bp specific fragment of glt A gene in total nucleic acid was amplified and the positive amplification products were sequenced. The sequences of phylogenetic tree were analyzed by Mega 5.0 software and GenBank. Results A total of 644 mice were caught, belonging to 3 orders, 5 families and 8 genera. Among them, Apodemus agrarius was the predominant mouse species, accounting for 42.70% (275/644) of the total number of captured monkeys, followed by the red-backed Pinghu Microtus fortis, accounting for 27.64% % (178/644) and 21.12% (136/644). A total of 31 mice were found to be infected with typhus, the positive rate was 4.81%, which belonged to 5 rodents. Among them, the infection rate of Microtus fortis was 9.56% (13/136), other rodents The infection rate of animals was relatively low, but there was no significant difference in the infection rates among the various species (P = 0.156). The infection rate of female mice (10/168, 5.95%) was higher than that of male mice (21/476, 4.41%), but the difference was not statistically significant (χ2 = 0.643, P = 0.423). The body weight mean (38.2548 g) of the typhus group was not significantly different from that of the non-infected group (32.2271 g) (F = 1.633, P = 0.202). The infection rates of typhus among mice in different time were statistically different (χ2 = 191.419, P = 0.000). Rickettsia in the same group was identical in evolutionary clade, the similarity was> 99.98%, which was most similar to the American strain CP003398, the similarity was> 95.83%. Conclusion There is a typhus Rickettsia infection in the rat population of Heishuizidao Island, and the seasonal variation exists in the infection status, which provides the basis for the scientific development of the black blind island.