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研究了中华鲟幼鱼经不同浓度铜(Cu2+)水体(低浓度组(0.40μg/L)、中浓度组(0.89μg/L)、高浓度组(2.00μg/L)、对照组(0.00μg/L))暴露60d后,铜在幼鱼不同组织中的积累以及对幼鱼生长、消化酶活性的影响.结果显示,随着水体Cu2+浓度的增加,幼鱼体质量显著减轻,低浓度组与对照组(无Cu2+水体)无显著性差异(P>0.05),中高浓度组与对照组均有显著性差异(P<0.05).对照组中华鲟幼鱼8种组织中铜含量的顺序从高到低依次为肝脏>鳃>背骨板>肌肉>皮肤>脊索>软骨>消化道;经不同浓度Cu2+水体暴露60d后,随着Cu2+浓度的升高,8种组织中铜含量与对照相比均显著增加(P<0.05),肝脏中铜的含量仍最高;消化道中铜含量增加最多,低浓度组是对照组的21.41倍,高浓度组是对照组的58.58倍;表明肝脏和消化道是Cu2+主要的累积和代谢器官.随着Cu2+浓度升高,消化道蛋白酶、淀粉酶的活性与对照组相比显著下降(P<0.05);脂肪酶活性则随着Cu2+浓度的升高而升高,低浓度组与对照差异不显著,中高浓度组与对照组均有显著性差异(P<0.05).表明Cu2+对蛋白酶和淀粉酶的活性有抑制作用,对脂肪酶活性有促进作用.代谢酶活性的变化可以灵敏地反映Cu2+的胁迫程度和毒性.
The effects of different concentrations of copper (Cu2 +) water (0.40μg / L, 0.89μg / L), high concentration group (2.00μg / L) / L)), the accumulation of copper in different tissues of juvenile fish and the effects on the growth and digestive enzyme activity of juvenile fish after 60 days of exposure.The results showed that with the increase of water Cu2 + concentration, the body weight of juvenile fish decreased significantly, (P> 0.05), but there was significant difference between the medium and high concentration groups and the control group (P <0.05) .The order of the copper content in 8 tissues of juvenile Acipenser sinensis High to low followed by liver> gills> vestibule> muscle> skin> notochord> cartilage> digestive tract; after exposure to different concentrations of Cu2 + water for 60 days, with the increase of Cu2 + concentration, (P <0.05). The content of copper in the liver was still the highest. The content of copper in the digestive tract increased most, 21.41 times in the low concentration group and 58.58 times in the high concentration group, indicating that the liver and digestive tract were The main accumulation and metabolic organs of Cu2 + .With the increase of Cu2 + concentration, the activities of digestive tract protease and amylase were significantly decreased compared with the control group ( P <0.05). The activity of lipase increased with the increase of Cu2 + concentration, while the concentration of Cu2 + in the medium and high concentration groups was significantly different from the control group (P <0.05) And amylase activity inhibited, and promoted the activity of lipase.Changes in metabolic enzyme activity can sensitively reflect the degree of Cu2 + stress and toxicity.