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目的了解2007-2008年北京地区柯萨奇病毒A组16型(Coxsackievirus A16,CA16)流行情况及其种系进化规律和基因分型。方法收集北京地区2007-2008年133例手足口病病例临床标本,从中筛选CA16毒株,扩增部分毒株VP1区并进行序列测定和分析。结果共筛选得到39例45份CA16阳性标本,测定其中16株病毒的VP1区核苷酸序列,其同源性为91.43%～98.65%,氨基酸同源性为97.98%～100%。该16株病毒与GenBank中选取的24株CA16参考株的核苷酸同源性为78.13%～98.65%,氨基酸同源性为89.9%～100%。16株北京分离株中的9株与近年多数中国大陆分离株形成进化树上亲缘关系较近的一群,其余7株与我国台湾及周边国家的一系列分离株亲缘关系较近。结论CA16为北京地区手足口病的主要致病原之一,本地区毒株同源性较高,同GenBank中24株CA16参比毒株序列进行系统进化分析显示其属于B基因群,16株北京分离株在系统进化树上有分别归属于两支的趋势。
Objective To understand the prevalence and evolutionary patterns and genotyping of Coxsackievirus A16 (CA16) in Beijing from 2007 to 2008. Methods A total of 133 cases of hand, foot and mouth disease were collected from Beijing in 2007-2008. The CA16 strains were screened and some VP1 strains were amplified and sequenced. Results A total of 45 CA16 positive samples were screened. The nucleotide sequence of the VP1 region of 16 viruses was determined. The homology was 91.43% -98.65%, and the amino acid homology was 97.98% -100%. The nucleotide sequences of these 16 viruses were 78.13% -98.65% with homology of 24 strains of CA16 selected in GenBank, and their amino acid identities were 89.9% -100%. Nineteen out of 16 Beijing isolates were closely related to the phylogenetic trees of most Chinese mainland isolates in recent years. The remaining 7 isolates were closely related to a series of isolates from Taiwan and its neighboring countries in China. Conclusion CA16 is one of the major causative pathogens of HFMD in Beijing and its homology is high. Phylogenetic analysis of 24 CA16 reference strains in GenBank showed that it belonged to B gene group, 16 Beijing isolates have phylogenetic relationships with two phylogenetic trees respectively.