论文部分内容阅读
利用经典遗传学的杂文－互交方法将昆明（ＫＭ）小鼠携带的先天性骨─肾畸形综合征致病基因１ｄ导入遗传背景已知的Ｃ５７ＢＬ／６近交品系小鼠中，建立Ｃ５７ＢＬ／６－１ｄ同源导入近交系。本实验随机挑选第六、七循环Ｃ５７ＢＬ／６－１ｄ动物共１２只，采用蛋白质和同工酶电泳方法对位于共九对染色体上的１４个生化标记基因位点进行检测，结果表明，第六循环的Ｃ５７ＢＬ／ｅ－１ｄ在Ｅｓ－１和ＧＰｄ－１位点的等位基因为杂合型，至第七循环，Ｃ５７ＢＬ／６－１ｄ动物的１４个位点全部为纯合子，与Ｃ５７ＢＬ／６基因型一致。
The classical genetics essay - reciprocal method to Kunming (KM) mice carrying congenital bone-kidney malformation syndrome-induced gene 1d introduced into the genetic background of C57BL / 6 inbred strains of mice known to establish C57BL / 6-1d homologous introduction of inbred lines. In this experiment, we randomly selected 12 C57BL / 6-1d animals in the sixth and seventh cycles and detected 14 biochemical marker loci in nine pairs of chromosomes using protein and isozyme electrophoresis. The results showed that the sixth The circulating alleles of C57BL / e-1d at Es-1 and GPd-1 loci were heterozygous. By the seventh cycle, the 14 loci of C57BL / 6-1d animals were all homozygous and the C57BL / 6 genotypes consistent.