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以葡萄糖为碳源合成荧光碳点;以巯基琥珀酸为稳定剂,合成CdTe量子点。通过紫外吸收光谱和荧光发射光谱对二者的荧光性能进行表征。又以毕赤酵母作为指示生物,考察了延滞期的毕赤酵母分别与荧光碳点和量子点共培养后的生长曲线,并利用血球计数板法对培养至25h的毕赤酵母细胞进行计数。研究结果表明:荧光碳点对毕赤酵母的生长抑制作用不明显,即使在高浓度(14.4 mmol/L)下,也基本不影响毕赤酵母的生长;而CdTe量子点必须控制在很低的浓度(5.1μmol/L),才表现出低的细胞毒性。
Glucose as a carbon source to synthesize fluorescent carbon dots; mercapto succinic acid as a stabilizer to synthesize CdTe quantum dots. The fluorescence properties of the two were characterized by ultraviolet absorption spectroscopy and fluorescence emission spectroscopy. Pichia pastoris as an indicator organism, the lag phase of Pichia pastoris respectively with the fluorescent carbon spots and quantum dots after co-cultured growth curve, and the use of hematology plate count cultured to 25h Pichia cells were counted. The results showed that the fluorescent carbon spots had no significant effect on the growth of Pichia pastoris, and did not affect the growth of Pichia pastoris even at high concentration (14.4 mmol / L). However, the CdTe quantum dots had to be controlled at very low Concentration (5.1μmol / L), showed low cytotoxicity.