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应用铁氰化钾还原法、Fenton法、邻苯三酚自氧化实验及DPPH自身显色实验分别检测苦参多糖的还原能力及对羟基自由基、超氧阴离子自由基和·DPPH自由基的清除能力.研究了苦参多糖对高糖诱导的红细胞氧化应激的影响,考察苦参多糖对红细胞中超氧化物歧化酶(Supemx-ide dismutase,SOD)、过氧化氢酶(Catalase,CAT)和谷胱甘肽还原酶(Glutathione peroxidase,GSH)活性及红细胞中活性氧(Reactive oxygen species,ROS)含量的影响.结果表明,苦参多糖具有良好的还原能力和清除自由基能力,且与多糖浓度呈计量依赖关系(n=3,p ＜0.05),但这一作用弱于阳性对照Vc.苦参多糖可以提高红细胞中SOD、CAT和GSH的活性,清除活性氧,从而改善由高糖诱导的红细胞的氧化应激状态.因此,根据糖尿病并发症机制,苦参多糖可能为中药苦参中抑制糖尿病氧化应激的活性物质,对糖尿病及其并发症的预防和治疗具有重要意义.“,”Effect of Sophora flavescens polysaccharide (SFP) on maintaining redox equilibrium in erythrocyte was studied.First,the reducing capacity and scavenging abilities for hydroxyl radical,superoxide anion radical and · DPPH radical were estimated with the system of potassium ferricyanide,H2O2/Fe2+,pyrogallol auto-oxidation and DPPH chromogenic,respectively.Then,we determined the effects on activities of supemxide dismutase,glutathione peroxidase and catalase,which were related to redox equilibrium in erythrocyte.The total content of ROS in erythrocyte was also determined.We found that SFP had a good reducing capacity and free radical scavenging ability with a dose-dependent manner,but which was weaker than Vc group (n =3,p ＜ 0.05).SFP increased SOD,CAT and GSH activities under the condition of high glucose concentration in erythrocyte.SFP attenuated production of ROS in erythrocyte.Hence,according to diabetic complications mechanism,SFP might be effective for preventing and treating diabetic complications.