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作者研究了去甲肾上腺素(NA)在人胎盘、脐动脉、脐静脉、华顿氏胶(Wharton’s jelly)和胎膜中的代谢灭活。材料取自16名正常妊娠、自然分娩的妇女,于产后20分钟内分别取胎盘、胎膜、脐动脉、脐静脉和华顿氏胶各0.5g切成0.2mm原的组织片置玻片上,在Krebs-Ringer碳酸氢盐缓冲液、氧气与二氧化碳(19∶1)的混合气体中于37℃下培养。然后加入〔1-~3H〕NA(比放活性10.1-10.9 Ci/mmol)使最后浓度为10~(-7)M。孵育30分钟后以高氯酸中止孵育,组织片经匀浆、离心后收集上清液用
The authors studied the metabolic inactivation of norepinephrine (NA) in human placenta, umbilical artery, umbilical vein, Wharton’s jelly and fetal membranes. Materials from 16 normal pregnancy, women of natural childbirth, were taken within 20 minutes after delivery, placenta, fetal membranes, umbilical artery, umbilical vein and Wharton’s gum each 0.5g cut into 0.2mm original tissue slide glass, Incubate at 37 ° C in a mixture of Krebs-Ringer bicarbonate buffer, oxygen and carbon dioxide (19: 1). Then [1- 3 H] NA (specific activity 10.1-10.9 Ci / mmol) was added to bring the final concentration to 10 -7 M. Incubation 30 minutes after the incubation with perchloric acid suspension, tissue pieces were homogenized, the supernatant was collected after centrifugation