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目的 :比较两种不同方式介导IL 12真核表达载体抑制肝移植瘤生长的效果。方法 :利用鼠肝癌细胞系H2 2建立肝移植瘤模型 ,以脂质体介导IL 12质粒腹腔注射或直接瘤内注射给荷瘤鼠 ,每周 1次 ,共 2次。治疗后每周两次测量瘤体大小 ,取血利用ELISA法检测血清中IL 12及IL 2的含量变化 ;MTT法检测NK细胞杀伤活性。结果 :直接瘤内注射组瘤体积明显小于腹腔注射组 (P <0 .0 5 ) ,直接瘤内注射组血清IL 12、IL 2浓度有明显的周期变化 ,但二者NK细胞杀伤率无明显差异。结论 :脂质体介导直接瘤内注射IL 12真核表达载体可有效抑制肝移植瘤的生长。
OBJECTIVE: To compare the effects of two different ways of inhibiting the growth of liver xenografts in IL-12 eukaryotic expression vector. Methods: The model of liver xenograft was established by murine hepatocellular carcinoma cell line H2 2. The tumor-bearing mice were injected intraperitoneally with IL-12 plasmid or injected intratumorally with liposomes twice a week for 2 times. Tumor size was measured twice a week after treatment. The levels of IL-12 and IL-2 in serum were detected by ELISA. The cytotoxic activity of NK cells was detected by MTT assay. Results: The tumor volume of direct intratumoral injection group was significantly smaller than that of intraperitoneal injection group (P <0.05). The concentration of IL-12 and IL-2 in direct intratumoral injection group had obvious periodic changes, but the NK cell killing rate of the two groups had no obvious difference. Conclusion: Liposome-mediated direct intratumoral injection of IL-12 eukaryotic expression vector can effectively inhibit the growth of liver xenografts.