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将狂犬病病毒糖蛋白ｃＤＮＡＢｇｌⅡ（１．６７ｋｂ）片段分别正向插入ｐｍＭＴ－１ＢｇｌⅡ位点和ｐＭＴ０１０／Ａ＋ＢａｍＨＩ位点，构建重组质粒ｐｍＭＴ－Ｒｇｐ和ｐＭＴ０１０／Ａ＋－Ｒｇｐ，通过磷酸钙共沉淀法转染ＢＨＫ－２１细胞，经ＰＣＲ、打点杂交及ＥＬＩＳＡ检测，证明糖蛋白基因发生了整合并获得表达。以该表达载体给小鼠肌肉内注射２～３次，采其注射前、后双份血清，经ＥＬＩＳＡ检测证明，注射了表达载体的小鼠，血清中病毒特异性抗体明显升高，表明狂犬病病毒糖蛋白ｃＤＮＡ在小鼠体内表达后，刺激机体产生了抗该糖蛋白特异性抗体
Rabies virus glycoprotein cDNABgl Ⅱ (1.67kb) fragment was inserted into the pmMT-1BglⅡ site and pMT010 / A + BamHI site respectively, and the recombinant plasmids pmMT-Rgp and pMT010 / A + -Rgp were constructed and transfected into BHK by calcium phosphate coprecipitation -21 cells by PCR, dot blot hybridization and ELISA test to prove that the glycoprotein gene integration and expression. Mice were intramuscularly injected with this expression vector for 2 or 3 times. Before and after injection, duplicate sera were collected and tested by ELISA. The mice injected with the expression vector had a significantly higher serum specific antibody, indicating that rabies Expression of viral glycoprotein cDNA in mice stimulates the body to produce antibodies specific to the glycoprotein