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水稻是一种很重要的模式植物,其基因组框架图的完成将对植物生物学和遗传进化学等学科的研究做出巨大贡献.目前,水稻科研工作者们在绘制水稻完成图谱的同时,也正在对水稻中基因和非编码区序列进行着深入的研究.非编码RNA在生物系统中有着很重要的作用.小RNA是非编码RNA的一种,我们在水稻基因组中寻找已知小RNA序列,并且在拟南芥、玉米、酵母、线虫、老鼠和猪这6个物种中一一进行比对,结果在552个小RNA的数据库中找到160个小RNA,它们存在于水稻的108个Scaffold中,其中绝大部分(99.41％)都位于基因预测的内含子区.19个小RNA只存在于水稻基因组中.发现了两段U14小RNA保守片段,一段是位于同系列的5个小RNAZMU14SNR9(s)中,它们只出现在3个植物物种上,其中86％序列都是和水稻、拟南芥、玉米重复的序列;另一段保守的小RNA是XLHS7CU14,它出现在除猪以外的其他6个物种中.所有这些结果显示小RNA在植物和动物之间并没有明显的界限.
Rice is a very important model plant and the completion of its genome map will make a great contribution to the research of plant biology and genetic evolution etc. At present, Non-coding RNAs are playing a very important role in biological systems.MicroRNAs are a kind of non-coding RNAs, we are looking for the known small RNA sequences in rice genome, And aligned in one of six species of Arabidopsis, corn, yeast, nematode, mouse and pig. As a result, 160 small RNAs were found in a database of 552 small RNAs, which are present in 108 Scaffolds of rice , Most of which (99.41%) were located in the intron region predicted by the gene .19 small RNAs existed only in the rice genome. Two U14 small RNA conserved fragments were found, one was located in the same series of five small RNAZMU14SNR9 (s), they occur only on three plant species, of which 86% are sequences that repeat with rice, Arabidopsis, and maize; the other conserved small RNA is XLHS7CU14, which appears in other than pigs 6 things In. All of these results show that small RNA between plants and animals, and no clear boundaries.