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运用ｍＲＮＡ差异显示（ＤＤ－ＰＣＲ）技术，通过２６对锚定及任意引物组合显示基因的差异表达情况，分离了７个表达有差异的片段．其中３个片段的相关基因属于对Ｎ－甲基－Ｎ′－硝基－Ｎ－亚硝基胍（ＭＮＮＧ）处理的初级反应基因，２个属于次级反应基因．另有２个片段的差异表达仅在蛋白合成抑制剂环己亚胺（ＣＨＭ）合并ＭＮＮＧ处理时才出现．反向缝隙印迹杂交分析印证了２个片段在ＤＤ－ＰＣＲ中发现的改变，同时分析的本实验室分离的２５个片段中，８个片段的变化被验证与ＤＤ－ＰＣＲ中的改变一致．序列分析结果显示这些片段与许多基因有高同源性，其中包括一些参与信息传导的基因．
Using differential display of mRNA (DD-PCR) technology, 26 pairs of anchored and arbitrary primers were used to display the differential expression of genes, and 7 differentially expressed fragments were isolated. The related genes of the three fragments belonged to the primary reaction genes for the treatment of N-methyl-N’-nitro-N-nitrosoguanidine (MNNG), and two of them belonged to the secondary reaction genes. The differential expression of the other two fragments appeared only when the protein synthesis inhibitor cycloheximide (CHM) was combined with MNNG treatment. Reverse slit-blot hybridization analysis confirmed the changes found in the two fragments in DD-PCR. The simultaneous analysis of the changes in the eight fragments isolated in this laboratory was validated in line with the changes in DD-PCR. Sequence analysis showed that these fragments have high homology with many genes, including some genes involved in information transmission.