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在微乳液体系中合成荧光二氧化硅纳米颗粒(FSNPs),然后对其表面进行氨基修饰,之后再连接抗体,应用高分辨透射电镜(HR-TEM),原子力显微镜(AFM),Zeta-电位分析仪,IR,荧光显微镜及激光共聚焦显微镜等多种表征工具对合成的颗粒和后续的修饰进行了系统分析.研究表明,FSNPs在溶液中呈现单分散状态,但是在表面修饰了氨基之后(NH2-FSNPs),由于表面平均电位以及氨基间氢键的形成,致使颗粒严重聚集;适当的pH环境可以改善颗粒的分散状态;利用氨基修饰的荧光二氧化硅纳米颗粒(NH2-FSNPs)连接抗体识别脐血来源的间充质干细胞(MSCs)表面的蛋白CD44,从而实现对间充质干细胞荧光成像分析.结果表明,识别效果良好,为荧光二氧化硅纳米颗粒在干细胞领域的应用奠定了基础.
In the microemulsion system, fluorescent silica nanoparticles (FSNPs) were synthesized, then the amino groups were modified on the surface of the nanoparticles, and then the antibody was added to the surface of the nanoparticles. High resolution transmission electron microscopy (HR-TEM), atomic force microscopy (AFM), Zeta- IR, fluorescence microscopy and confocal laser scanning microscopy and other characterization tools for the synthesis of the particles and the subsequent modification of the system analysis.Studies showed that FSNPs monodispersed in solution, but after the surface modification of the amino (NH2 -FSNPs), due to the formation of surface average potential and the formation of hydrogen bonds between amino groups, resulting in serious aggregation of particles; appropriate pH environment can improve the dispersion of particles; using amino-modified fluorescent silica nanoparticles (NH2-FSNPs) (CD44) on the surface of mesenchymal stem cells (MSCs) derived from umbilical cord blood in order to realize the fluorescence imaging analysis of mesenchymal stem cells.The results show that the recognition effect is good, which lays the foundation for the application of fluorescent silica nanoparticles in the field of stem cells.