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用胶原酶连续灌注大鼠肝脏而获得形态典型、分离率高的肝细胞。用灌注法分离出来的肝细胞成活率高达90％,ATP含量在正常生理范围(8～10n mol/mg干重细胞),肝细胞具有葡萄糖异化作用的酶系能利用丙酮酸、乳酸为底物合成葡萄糖。在MEM培养剂中培养的肝细胞属均匀的单层上皮型细胞,在2～3天内保持原有形态,为非分裂
Continuous collagenase perfusion rat liver morphology and high isolation rate of liver cells. The survival rate of hepatocytes isolated by perfusion method was as high as 90%. The ATP content in the normal physiological range (8 ~ 10n mol / mg dry weight cells) and the hepatic cells with glucose dissimilation could utilize pyruvate and lactate as substrates Glucose synthesis. The hepatocytes cultured in MEM medium are uniform monolayer of epithelial cells that remain in their original form within 2 to 3 days and are non-dividing